Confocal laser scanning microscopy

Course code MEKM01F

Confocal laser scanning microscopy is mainly used in biomedical sciences for high-resolution analysis of cell and tissue structures labeled with fluorescent markers. It is the prevailing microscope technique for ”deeper” analysis at cellular and sub-cellular levels. High resolution is achieved by scanning the sample with a finely focussed laser beam, and exclusion of out-of-focus fluorescence by a confocal aperture. The acquired images can be used for analysis and processing, e.g. 3D-reconstruction, of the labeled structures.

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Time: week 48

Contact: Sebastian Wasserström sebastian.wasserstrom@med.lu.se