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Microscopes

The aim of this list is to supply useful information about tools around Lund University.

The idea is that if you are interested in trying out a technique or need to talk to someone who has knowledge of a particular method, here you can find it. Some of the tools are "public" and can be used by anyone by paying by the hour or similar whereas some tools belong to specific research groups. If you are interested, the easiest way to proceed would be to approach one of the ADMIRE members listed as users and they can tell you more about how to get access to the tool and what it is capable of.

This list is only as useful as we make it; if you are using a tool which is not listed, please drop us a line with some information and we'll add it. You can either send us an email or fill out our form.
If you are using a tool listed, we can add you as a user as well if you let us know.

We hope you will put this list to good use.



Optical Microscopy

ToolDescriptionLocationUsersAccessResponsible

Zeiss LSM510 (laser confocal)

7 laser lines, 15 emission filters. Spectral detector ("lambda") as well as two photomultiplier tubes. Equipped with stage incubator (CO2, humidifier, 37°C)

More info.

Biology Department

Pay by the hour, with or without technician.

Anyone (after training). 

~50% occupancy

More info.

Ola Gustafsson

+46 (0)46 222 93 43

Leica SP8 DLS (inverted microscope) 

Light sheet and a confocal microscope; light source: five lasers with wavelength (405 nm, 488 nm, 514 nm, 552 nm, 663 nm); detectors: two PMT:s, HyD GaAsP-spectral detector, PMT for transmitted light; objectives confocal imaging: 10x/0.3, 20x/0.75 IMM, 25x/0.95W, 63x/1.4 Oil, 63x/1.3 GLYC; objectives and mirrors for light sheet microscopy: imaging 25x/0.95W DLS, 10x/0.3W DLS, illumination 2,5x/0.07, 5x/0.15, DLS TwinFlect 2.5mm, DLS TwinFlect 5mm

More info.

Biology Department

Pay by the hour, with or without technician.

Anyone (after training). 

~50% occupancy

More info. 

Ola Gustafsson

+46 (0)46 222 93 43

Nikon TE2000 (fluorescence microscope) Many different objectives (from 0.5x to 100x oil) Lots of extra equipment e.g. TIRF, high speed camera, spinning disc confocal , DIC, dark field ,FRAP. Department of Physics, Solid State Physics C. Niman,
S. Holm,
K. Adolfsson,
H. Persson
Via collaboration, ~50% occupancy Jonas Tegenfeldt
PHI AB M3 (phase holographic microscope) Phase holographic microscopy for live cell imaging. Microscope in incubator (37C, no atmosphere => use closed culture flask). Objectives: 10-20x. Biology Department, Functional Zoology H. Persson
J. Stegmayr
X. Huang
Via collaboration, ~100% occupancy Stina Oredsson
Zeiss axio imager (deconvolution) A system for wide-field microscopy with high resolution, high sensitivity, and equipped for 3D imaging, deconvolution, live cell time-lapse, photoactivation, photobleaching etc (optimised for bacterial cell biology, but applicable to many other types of samples):
Zeiss AxioImager.Z1 (upright), XY scanning table, X-Cite illumination, Hamamatsu C9100-02 EM-CCD camera, Volocity 3DM software, Micropoint Laser system.
Biology Department, Molecular Cell BiologyVia collaborationKlas Flärdh
LSM700 Axioimager M2 (upright) LSM700 Axioimager M2 (upright), motorized stage incl module for tiles (stitching) zen 2012 Lasers 405, 488, 555, 635 nm DIC etc Immunology, BMC section D14 Immunology section and external users Fee and open for everybody after training.
30% Occupancy
Lena M Svensson
Zeiss AxiovisionZeiss Axiovison (inverted) fluorescence microscope with incubator motorized stage, X-cite (light source), filters: ex350/50 em 460/50 ex 470/40 em 525/50 ex566/10 em610/60 samt ex640/30 em Immunology, BMC section D14 Immunology section Fee and open for everybody after training.
90% Occupancy
Lena M Svensson
TIRF/FLIM microscope Zeiss observer Z1 (inverted) Incubator, motorized TIRF Slider, definite focus, Lasers: 405, 445, 488, 515, 561, 640, Evolve EMCCD (512x512) CSU-X1 A Spinning disc, Vector for photomanipulation (FRAP, PALM etc), mSwitcher, FLIM with PIMax-4 camera.
Software: Slidebook 5.5
Plenty of objectives, incl 100x Na1.46 DIC (TIRF), Antiflex x63 NA 1.25 for IRM as well as water 63 x NA 1.3
Immunology, BMC D14 Leuokocyte migration Via collaboration, Lena M Svensson
Nikon A1+ (Laser Scanning Confocal) 405, 488, 561 and 640 lasers, 4 detectors 2 of which high-sensitivity GaAsP, Nikon Ti-E motorized inverted research microscope with constant perfect focus mechanism (PFS3), various objectives LBIC, BMC D11, LU Core Facility Open, see LBIC website for more information Sebastian Wasserström
Nikon N-STORM (Super Resolution) 405, 488, 561 and 647 lasers, iXON 897 EMCCD, Nikon Ti-E motorized inverted research microscope with constant perfect focus mechanism (PFS3), Motorized TIRF, 3D STORM imaging 20nm x/y, 50nm z resolution, 100x 1.49NA TIRF objective among others LBIC, BMC D11, LU Core Facility Open, see LBIC website for more information Sebastian Wasserström
Nikon Ti-E with TIRF 405, 457, 477, 488, 514, 561 and 640 lasers for TIRF, Solid-state Spectra-X with 405, 457, 488, 514, 561 and 640 lines for EPI-FL, iXON Ultra 897 EMCCD, Nikon Ti-E motorized inverted research microscope with constant perfect focus mechanism (PFS3), Motorized TIRF, Real-time triggering for fast temporal TIRF/EPI-FL experiments, Stage-top incubator with temperature control (3°C above ambient to 45°C), 100x 1.49NA TIRF objective among others LBIC, BMC D11, LU Core Facility Open, see LBIC website for more information Sebastian Wasserström
Nikon NIS-Elements AR offline workstation Nikon NIS-Elements advanced research software package including: 3D measurement, Co-localization and ratio-imaging algorithms, advanced image analysis tools, deconvolution algorithms, etc. LBIC, BMC D11, LU Core Facility Open, see LBIC website for more information Sebastian Wasserström
TIRF microscopeCustom made setup for high-resolution fluorescence imaging capable of single molecule studies, fluorescence recovery after photobleaching (FRAP) and total internal reflection fluorescence (TIRF), imaging at two wavelengths simultaneously (488 nm and 638 nm excitation) with micropipette manipulation

Department of Chemistry, Physical Chemistry

Victoria JunghansMore info, here.Peter Jönsson
Nikon Ti-E Live-cell system Solid-state Spectra-X with 405, 457, 488, 514, 561 and 640 lines for EPI-FL, iXON Ultra 897 EMCCD, Nikon Ti-E motorized inverted research microscope with constant perfect focus mechanism (PFS3), Real-time triggering for fast temporal EPI-FL experiments, Cage incubator with CO2 (0-18%) and temperature (3°C above ambient to 45°C) control with humidifier, various objectives Wallenberg Neuroscience Center, LU Jia-Yi Li Via collaboration Jia-Yi Li


Electron Microscopy

ToolDescriptionLocationUsersAccessResponsible
Zeiss LEO1560 (SEM) Standard SEM, 2nd e- and BS detectors Department of Physics Lund Nanolab Most ADMIRE members from Solid State Physics Need cleanroom access.
Occupancy ~100%
Ivan Maximov for cleanroom access
FEI nanolab600 (FIBSEM) SEM. Equipped with ion beam for milling and deposition. Micromanipulator. Department of Physics Lund Nanolab Most ADMIRE members from Solid State Physics Need cleanroom access.
Occupancy ~80%
Ivan Maximov for cleanroom access
Hitachi SU8010 (SEM) SEM. 2nd e- and BS detectors. Cold trap. Department of Physics Lund Nanolab Most ADMIRE members from Solid State Physics Need cleanroom access.
Occupancy ~50%

Ivan Maximov for cleanroom access

 

Hitachi SU3500 (SEM) Tungsten filament, high vac, low vac, 2nd e- and backscatter detectors, low vac 2nd e- detector. More info. Biology Department Pay by the hour, with or without technician.
Help with sample prep available.

Anyone  (after training).

More info.

Ola Gustafsson

+46 (0)46 222 93 43

JEOL 1400 Plus (TEM) TEM, 120kV, LaB6 Filament, CMOS camera
More info.
Biology Department Pay by the hour, with or without technician.

Help with sample prep available.
More info.
 

Ola Gustafsson

+46 (0)46 222 93 43

JEM-2200FS A transmission electron microscope that is specially designed for cryo-TEM, low dose imaging and tomography. More info, here.nCHREM, KILU Mainly from KILU but also other LU users as well as external users. Open facility. Hourly charge with or without (for qualified users) operator. Sample preparation available. Anna Carnerup
JEOL 3000F Analytical TEM with a field-emission electron source. Video rate camera, 2 x 2 k CCD camera. STEM mode with high-angle annular detector. SDD based Oxford XEDS system. Gatan Imaging Filter (2 x 2 k) nm. Specialised holders. nCHREM, KILU Mainly from KILU but also other LU users as well as external users. Open facility. Hourly charge with or without (for qualified users) operator. Sample preparation available. Crispin Hetherington
JSM-6700F SEM. Field emission gun. Oxford XEDS system. nCHREM, KILU Mainly from KILU but also other LU users as well as external users. Open facility. Hourly charge with or without (for qualified users) operator. Sample preparation available. Anna Carnerup, Daniel Jacobsson


Scanning Probe Microscopy

ToolDescriptionLocationUsersAccessResponsible
JEOL JSTM 4500 XT (STM) STM in ultrahigh vacuum (UHV). Liquid-Helium cooling option down to 20 K. Standard preparation techniques.

 

 

Department of Physics, Synchrotron Radiation Research

More info about the Lund University Scanning Probe Microscopy Facility (LUSP) be found here.



ADMIRE members from Synchrotron Radiation Research

via collaboration Anders Mikkelsen,
Rainer Timm
Omicron VT XA STM UHV-STM at variable temperatures (room temperature to 800°C), with a qPlus option for non-contact AFM and extra electrical contacts to the sample. Standard preparation techniques. Anders Mikkelsen
Omicron VT XA STM UHV-STM at variable temperatures with liquid-Helium cooling option (50 K – 1100 K. Anders Mikkelsen, Edvin Lundgren, Joachim Schnadt
Omicron 1 STM UHV-STM with extended preparation techniques, mass spectrometry, Auger spectroscopy, LEED

Edvin Lundgren,Jan Knudsen

Sigma Infinity LT-STMUHV-STM at low temperatures (< 10K, closed-cycle cooling), with a qPlus option for non-contact AFM and extra electrical contacts to the sample for operando device characterizationAnders Mikkelsen, Rainer Timm

Unisoku SEUL STM

small, light-weight, open-geometry STM for flexible environments, with q-Plus AFM functionalityAnders Mikkelsen, Rainer Timm
Nanosurf NaioAFMsmall, flexible AFM

Edvin Lundgren, Rainer Timm

JPK NanoWizard IIAFM for operation in air or liquid environment, with c-AFM and KPFM functionalitybelongs to the Division of Solid State Physicsvia collaboration

Rainer Timm, Dan Hessman