lunduniversity.lu.se

ADMIRE

Lund University

Denna sida på svenska This page in English

Tools being used by members of ADMIRE

The aim of this list is to supply useful information about tools around Lund University.

The idea is that if you are interested in trying out a technique or need to talk to someone who has knowledge of a particular method, here you can find it. Some of the tools are "public" and can be used by anyone by paying by the hour or similar whereas some tools belong to specific research groups. If you are interested, the easiest way to proceed would be to approach one of the ADMIRE members listed as users and they can tell you more about how to get access to the tool and what it is capable of.

This list is only as useful as we make it; if you are using a tool which is not listed, please drop us a line with some information and we'll add it. You can either send us an email or fill out our form.
If you are using a tool listed, we can add you as a user as well if you let us know.

We hope you will put this list to good use.




Optical Microscopy

Tool Description Location Users Access Responsible
Zeiss LSM510 (laser confocal) 7 laser lines, 15 emission filters. Spectral detector ("lambda") as well as two photomultiplier tubes. Equipped with stage incubator (CO2, humidifier, 37C)
More info.
Biology Department H. Persson,
K. Adolfsson,
J. Stegmayr
Anyone (after training). Pay by the hour.

~50% occupancy
More info.
Peter Ekström
Ola Gustafson
Dan-E Nilsson
Nikon TE2000 (fluorescence microscope) Many different objectives (from 0.5x to 100x oil) Lots of extra equipment e.g. TIRF, high speed camera, spinning disc confocal , DIC, dark field ,FRAP. Department of Physics / Solid State Physics C. Niman,
S. Holm,
K. Adolfsson,
H. Persson
Via collaboration, ~50% occupancy Jonas Tegenfeldt
PHI AB M3 (phase holographic microscope) Phase holographic microscopy for live cell imaging. Microscope in incubator (37C, no atmosphere => use closed culture flask). Objectives: 10-20x. Biology Department / Functional Zoology H. Persson
J. Stegmayr
X. Huang
Via collaboration, ~100% occupancy Stina Oredsson
Zeiss axio imager (deconvolution) A system for wide-field microscopy with high resolution, high sensitivity, and equipped for 3D imaging, deconvolution, live cell time-lapse, photoactivation, photobleaching etc (optimised for bacterial cell biology, but applicable to many other types of samples):
Zeiss AxioImager.Z1 (upright), XY scanning table, X-Cite illumination, Hamamatsu C9100-02 EM-CCD camera, Volocity 3DM software, Micropoint Laser system.
Biology Department / Molecular Cell Biology Via collaboration Klas Flärdh
LSM700 Axioimager M2 (upright) LSM700 Axioimager M2 (upright), motorized stage incl module for tiles (stitching) zen 2012 Lasers 405, 488, 555, 635 nm DIC etc Immunology, BMC section D14 Immunology section and external users Fee and open for everybody after training.
30% Occupancy
Lena M Svensson
Zeiss Axiovision Zeiss Axiovison (inverted) fluorescence microscope with incubator motorized stage, X-cite (light source), filters: ex350/50 em 460/50 ex 470/40 em 525/50 ex566/10 em610/60 samt ex640/30 em Immunology, BMC section D14 Immunology section Fee and open for everybody after training.
90% Occupancy
Lena M Svensson
TIRF/FLIM microscope Zeiss observer Z1 (inverted) Incubator, motorized TIRF Slider, definite focus, Lasers: 405, 445, 488, 515, 561, 640, Evolve EMCCD (512x512) CSU-X1 A Spinning disc, Vector for photomanipulation (FRAP, PALM etc), mSwitcher, FLIM with PIMax-4 camera.
Software: Slidebook 5.5
Plenty of objectives, incl 100x Na1.46 DIC (TIRF), Antiflex x63 NA 1.25 for IRM as well as water 63 x NA 1.3
Immunology, BMC D14 Leuokocyte migration Via collaboration, Lena M Svensson
Nikon A1+ (Laser Scanning Confocal) 405, 488, 561 and 640 lasers, 4 detectors 2 of which high-sensitivity GaAsP, Nikon Ti-E motorized inverted research microscope with constant perfect focus mechanism (PFS3), various objectives LBIC, BMC D11, LU Core Facility Open, see LBIC website for more information Sebastian Wasserström
Nikon N-STORM (Super Resolution) 405, 488, 561 and 647 lasers, iXON 897 EMCCD, Nikon Ti-E motorized inverted research microscope with constant perfect focus mechanism (PFS3), Motorized TIRF, 3D STORM imaging 20nm x/y, 50nm z resolution, 100x 1.49NA TIRF objective among others LBIC, BMC D11, LU Core Facility Open, see LBIC website for more information Sebastian Wasserström
Nikon Ti-E with TIRF 405, 457, 477, 488, 514, 561 and 640 lasers for TIRF, Solid-state Spectra-X with 405, 457, 488, 514, 561 and 640 lines for EPI-FL, iXON Ultra 897 EMCCD, Nikon Ti-E motorized inverted research microscope with constant perfect focus mechanism (PFS3), Motorized TIRF, Real-time triggering for fast temporal TIRF/EPI-FL experiments, Stage-top incubator with temperature control (3°C above ambient to 45°C), 100x 1.49NA TIRF objective among others LBIC, BMC D11, LU Core Facility Open, see LBIC website for more information Sebastian Wasserström
Nikon NIS-Elements AR offline workstation Nikon NIS-Elements advanced research software package including: 3D measurement, Co-localization and ratio-imaging algorithms, advanced image analysis tools, deconvolution algorithms, etc. LBIC, BMC D11, LU Core Facility Open, see LBIC website for more information Sebastian Wasserström
Nikon Ti-E Live-cell system Solid-state Spectra-X with 405, 457, 488, 514, 561 and 640 lines for EPI-FL, iXON Ultra 897 EMCCD, Nikon Ti-E motorized inverted research microscope with constant perfect focus mechanism (PFS3), Real-time triggering for fast temporal EPI-FL experiments, Cage incubator with CO2 (0-18%) and temperature (3°C above ambient to 45°C) control with humidifier, various objectives Wallenberg Neuroscience Center, LU Jia-Yi Li Via collaboration Jia-Yi Li




Electron Microscopy

Tool Description Location Users Access Responsible
Zeiss LEO1560 (SEM) Standard SEM, 2nd e- and BS detectors Department of Physics Lund Nanolab Most ADMIRE members from Solid State Physics Need cleanroom access.
Occupancy ~100%
Ivan Maximov for cleanroom access
FEI nanolab600 (FIBSEM) SEM. Equipped with ion beam for milling and deposition. Micromanipulator. Department of Physics Lund Nanolab Most ADMIRE members from Solid State Physics Need cleanroom access.
Occupancy ~80%
Ivan Maximov for cleanroom access
Hitachi SU8010 (SEM) SEM. 2nd e- and BS detectors. Cold trap. Department of Physics Lund Nanolab Most ADMIRE members from Solid State Physics Need cleanroom access.
Occupancy ~50%
Ivan Maximov for cleanroom access
Hitachi SU3500 (SEM) Tungsten filament, high vac, low vac, 2nd e- and backscatter detectors, low vac 2nd e- detector. More info. Biology Department   Pay by the hour, with or without technician.
Help with sample prep available.
More info.
Ola gustafsson
Dan-E Nilsson
JEOL 5600 (SEM) SEM, tungsten filament, High vac, low vac, 2nd e- and backscatter detectors. No longer under service contract, replaced by hitachi SU3500. More info. Biology Department Pay by the hour, with or without technician.
Help with sample prep available.
More info.
Ola Gustafsson
Dan-E Nilsson
JEOL 1230 (TEM) TEM, 120kV, Tungsten filament, Gatan Multiscan CCD.
More info.
Biology department   Pay by the hour, with or without technician.
Help with sample prep available.
More info.
Ola gustafsson
Dan-E Nilsson
Philips CM 120 BioTWIN Cryo TEM designed for cryo and low dose imaging. 120kV. LaB6 filament Gatan GIF 100 and digital multiscan CCD camera, Gatan 791. Oxford CT 3500 Cryoholder and transfer system. nCHREM, KILU Mainly from KILU but also other LU users as well as external users. Open facility. Hourly charge with or without (for qualified users) operator. Sample preparation available. Gunnel Karlsson
JEOL 3000F Analytical TEM with a field-emission electron source. Video rate camera, 2 x 2 k CCD camera. STEM mode with high-angle annular detector. SDD based Oxford XEDS system. Gatan Imaging Filter (2 x 2 k) nm. Specialised holders. nCHREM, KILU Mainly from KILU but also other LU users as well as external users. Open facility. Hourly charge with or without (for qualified users) operator. Sample preparation available. Reine Wallenberg
Gunnel Karlsson
JSM-6700F SEM. Field emission gun. Oxford XEDS system. nCHREM, KILU Mainly from KILU but also other LU users as well as external users. Open facility. Hourly charge with or without (for qualified users) operator. Sample preparation available. Gunnel Karlsson




Scanning Probe Microscopy

Tool Description Location Users Access Responsible
JEOL JSTM 4500 XT (STM) STM in ultrahigh vacuum (UHV). Liquid-Helium cooling option down to 20 K. Standard preparation techniques. Department of Physics, Synchrotron Radiation Research ADMIRE members from Synchrotron Radiation Research via collaboration Anders Mikkelsen,
Rainer Timm
Omicron VT XA STM UHV-STM at variable temperatures (room temperature to 800°C), with a qPlus option for non-contact AFM and extra electrical contacts to the sample. Standard preparation techniques. Department of Physics, Synchrotron Radiation Research ADMIRE members from Synchrotron Radiation Research via collaboration Anders Mikkelsen
Omicron VT XA STM UHV-STM at variable temperatures with liquid-Helium cooling option (50 K – 1100 K. Department of Physics, Synchrotron Radiation Research ADMIRE members from Synchrotron Radiation Research via collaboration Anders Mikkelsen, Edvin Lundgren, Joachim Schnadt
Omicron 1 STM UHV-STM with extended preparation techniques, mass spectrometry, Auger spectroscopy, LEED Department of Physics, Synchrotron Radiation Research ADMIRE members from Synchrotron Radiation Research via collaboration Edvin Lundgren